Downstream Bioprocessing

An Introduction

Training Agenda

This workshop aims to provide basic hands-on training in Introduction to downstream bio-processing, bio-molecular interaction kinetics. 

Dates: 13-16 November, 2018
Venue: CCAMP, GKVK Campus, Bellary Rd, Bangalore & JFWTC, Whitefield, Bangalore

Highlights:
1. Introduction to Bio-molecular interaction kinetics
2. Purification Techniques
3. Hands-on Training: Downstream

Course Fees:
Rs. 15,000 (+18% GST) for Academia
Rs. 20,000 (+18% GST) for Startups
Rs. 25,000 (+18% GST) for Companies

For more details:
Email to: proteintech2018@ccamp.res.in
Phone: 080 6718 5052; 080 2308 5052
Website: www.ccamp.res.in

Register Now!

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Day1

Start

End

Time

Type

Topic Name

9:00

9:30

0:30

Introduction to the course / Program / Objectives

9:30

10:15

0:45

Lecture

Introduction to chromatography

10:15

11:00

0:45

Lecture

Sample preparation

11:00

11:15

0:15

Break

11:15

12:15

1:00

Lecture

Purification Technique: Size Exclusion Chromatography

12:15

13:15

1:00

Practical

Exp-1: Size Exclusion Chromatography (desalting experiment)

13:15

14:15

1:00

Lunch

14:15

15:00

0:45

Lecture

Introduction to ÄKTA and UNICORN

15:00

15:15

0:15

Break

15:15

15:30

0:15

Results Discussion Exp-1

15:30

16:15

0:45

Lecture

Purification Technique: Ion Exchange Chromatography

16:15

17:00

0:45

Practical

Exp-2: Ion Exchange Chromatography (linear gradient)

17:00

17:15

0:15

Day Summary

17:15

Day End

Day2

Start

End

Time

Type

Topic Name

9:00

9:30

0:30

Result Discussion Exp-2

9:30

10:15

0:45

Practical

Exp-3: Ion Exchange Chromatography (gradient optimization)

10:15

11:00

0:45

Lecture

Purification Technique: Hydrophobic interaction Chromatography

11:00

11:15

0:15

Break

11:15

11:30

0:15

Result Discussion Exp-3

11:30

12:15

0:45

Practical

Exp-4: Hydrophobic interaction chromatography (linear gradient)

12:15

13:00

0:45

Lecture

Optimization Part-1

13:00

14:00

1:00

Lunch

14:00

14:15

0:15

Result Discussion Exp-4

14:15

15:00

0:45

Practical

Exp-5: Hydrophobic interaction chromatography (gradient optimization)

15:00

15:15

0:15

Break

15:15

16:00

0:45

Lecture

Optimization Part-2

16:00

16:45

0:45

Lecture

Purification Technique: Affinity Chromatography

16:45

17:00

0:15

Lecture

Day Summary

17:00

Day End

Day3

Start

End

Time

Type

Topic Name

9:00

9:30

0:30

Result Discussion Exp-5

9:30

10:15

0:45

Lecture

Purification Strategy

10:15

11:00

0:45

Lecture

Column Packing

11:00

11:15

0:15

Break

11:15

13:00

1:45

Practical

Column Packing and testing

13:00

14:00

1:00

Lunch

14:00

15:00

1:00

Lecture

Scale-up and fine tuning

15:00

15:15

0:15

Break

15:15

16:00

0:45

Lecture

Column and resin maintenance

16:00

16:45

0:45

Lecture

Introduction to regulatory

16:45

17:00

0:15

Lecture

Day Summary

17:00

Day End

Day 4

Start

End

Time

Type

Topic Name

9:00

9:30

0:30

Lecture

Introduction to Biacore

9:30

10:30

1:00

Lecture

Basic assay development

10:30

11:00

0:30

Practical

Introduction to Biacore T200 & Immobilization of CM5 chip

11:00

11:15

0:15

Break

11:15

12:15

1:00

Practical

Introduction to Biacore T200 & Immobilization of CM5 chip (Continues..)

12:15

13:15

1:00

Lunch

13:15

14:45

1:30

Practical

Set up Kinetic assay

14:45

15:30

0:45

Lecture

Basic Kinetics

14:45

15:00

0:15

Break

15:00

16:15

1:15

Practical

Review kinetics data, Kinetic analysis

16:15

16:45

0:30

Lecture

Maintenance

16:45

17:00

0:15

Summary

17:00

Day End